Supersplat—spliced RNA-seq alignment

  title={Supersplat—spliced RNA-seq alignment},
  author={Douglas W. Bryant and Rongkun Shen and Henry D. Priest and Weng-Keen Wong and Todd C. Mockler},
MOTIVATION High-throughput sequencing technologies have recently made deep interrogation of expressed transcript sequences practical, both economically and temporally. Identification of intron/exon boundaries is an essential part of genome annotation, yet remains a challenge. Here, we present supersplat, a method for unbiased splice-junction discovery through empirical RNA-seq data. RESULTS Using a genomic reference and RNA-seq high-throughput sequencing datasets, supersplat empirically… CONTINUE READING
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