Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology.

@article{Soukka2001SupersensitiveTI,
  title={Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology.},
  author={Tero Soukka and Juho P T Paukkunen and Harri H{\"a}rm{\"a} and Stefan L{\"o}nnberg and Hanne Lindroos and Timo L{\"o}vgren},
  journal={Clinical chemistry},
  year={2001},
  volume={47 7},
  pages={1269-78}
}
BACKGROUND The extreme specific activity of the long-lifetime fluorescent europium(III) chelate nanoparticles and the enhanced monovalent binding affinity of multivalent nanoparticle-antibody bioconjugates are attractive for noncompetitive immunoassay. METHODS We used a noncompetitive, two-step immunoassay design to measure free prostate-specific antigen (PSA). Europium(III) chelate nanoparticles (107 nm in diameter) were coated with a monoclonal anti-PSA antibody (intrinsic affinity, 6 x 10… CONTINUE READING

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