Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes.

@article{Miller2015SuperresolutionIO,
  title={Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes.},
  author={Helen Miller and Zhaokun Zhou and Adam J M Wollman and Mark C. Leake},
  journal={Methods},
  year={2015},
  volume={88},
  pages={81-8}
}
As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating dye YOYO-1 against minor groove binding dye SYTO-13, using a bespoke multicolor single-molecule fluorescence microscope. We used a full-length ∼49 kbp λ DNA construct possessing oligo inserts at either terminus allowing conjugation of digoxigenin and biotin at opposite ends for tethering to a glass coverslip surface… CONTINUE READING