Superoxide poisons mononuclear iron enzymes by causing mismetallation.

  title={Superoxide poisons mononuclear iron enzymes by causing mismetallation.},
  author={Mianzhi Gu and James A. Imlay},
  journal={Molecular microbiology},
  volume={89 1},
Superoxide (O(2)(-)) is a primary agent of intracellular oxidative stress. Genetic studies in many organisms have confirmed that excess O(2)(-) disrupts metabolism, but to date only a small family of [4Fe-4S] dehydratases have been identified as direct targets. This investigation reveals that in Escherichia coli O(2)(-) also poisons a broader cohort of non-redox enzymes that employ ferrous iron atoms as catalytic cofactors. These enzymes were inactivated by O(2)(-) both in vitro and in vivo… CONTINUE READING


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