Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter.

@article{Coleman2004Superior5H,
  title={Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter.},
  author={Tricia M. Coleman and Guocan Wang and Faqing Huang},
  journal={Nucleic acids research},
  year={2004},
  volume={32 1},
  pages={e14}
}
Transcription from the commonly used GTP- initiating T7 class III promoter phi6.5 frequently produces heterogeneous RNA at both 3' and 5' ends. We demonstrate here that RNA transcripts from the T7 class II promoter phi2.5 have superior 5' homogeneity over those from the phi6.5 promoter, with comparable total RNA yields. The overall homogeneity of RNA transcripts is improved to different degrees depending on RNA sequences, although transcription under phi2.5 does not affect the 3' heterogeneity… CONTINUE READING