Superhelical path of the DNA in the nucleoprotein complex that activates the initiation of phage phi 29 DNA replication.

@article{Serrano1993SuperhelicalPO,
  title={Superhelical path of the DNA in the nucleoprotein complex that activates the initiation of phage phi 29 DNA replication.},
  author={Manuel Serrano and Claude Gutierrez and M. Salas and J. M. Hermoso},
  journal={Journal of molecular biology},
  year={1993},
  volume={230 1},
  pages={248-59}
}
Initiation of bacteriophage phi 29 DNA replication is activated by protein p6, a viral double-stranded DNA-binding protein that forms a nucleoprotein complex at the viral replication origins. This complex consists of a DNA right-handed superhelix wrapped around a multimeric protein p6 core with protein p6 dimers regularly bound every 24 base-pairs (bp). In this paper, we have constructed a concatemer formed by direct repeats of a 24 bp sequence previously proposed to act as a signal for protein… CONTINUE READING