Supercoils in prokaryotic DNA restrained in vivo.

@article{Pettijohn1980SupercoilsIP,
  title={Supercoils in prokaryotic DNA restrained in vivo.},
  author={David E. Pettijohn and O W Pfenninger},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 3},
  pages={1331-5}
}
Cells of Escherichia coli containing the plasmid F were gamma-irradiated with various doses to introduce determined numbers of single-strand breaks in the F DNA. The cells were then incubated to permit repair of the breaks while DNA gyrase was inhibited with coumermycin to limit restoration of any relaxed supercoil. Repaired, covalently continuous F DNA was isolated and its superhelical density was measured by two different methods. Both indicated that a major part (50-60%) of the negative… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.
28 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 28 extracted citations

Similar Papers

Loading similar papers…