Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography.


Supercoiled plasmids are an important component of gene-based delivery vehicles, applied in new therapeutic strategies such as gene therapy or DNA vaccination. However, aiming at the general distribution of plasmid DNA (pDNA) therapeutics requires a procedure to easily and efficiently assess the purity and recovery yield of the supercoiled (sc) plasmid… (More)
DOI: 10.1016/j.chroma.2010.11.002


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