Superantigen gene complement of Streptococcus pyogenes—relationship with other typing methods and short-term stability

@article{Fries2012SuperantigenGC,
  title={Superantigen gene complement of Streptococcus pyogenes—relationship with other typing methods and short-term stability},
  author={Ana Fri{\~a}es and Francisco Ricardo Miranda Pinto and Catarina Silva-Costa and Mario Ulloa Ram{\'i}rez and Jos{\'e} Melo-Cristino},
  journal={European Journal of Clinical Microbiology & Infectious Diseases},
  year={2012},
  volume={32},
  pages={115-125}
}
The profiling of the superantigen (SAg) encoding genes has been frequently used as a complementary typing method for group A streptococci (GAS), but a confusing gene nomenclature and a large diversity of primers used in screening has led to some conflicting results. The aim of this work was to develop a polymerase chain reaction (PCR) method capable of efficiently amplifying all the known allelic variants of these genes, and to evaluate the congruence of this methodology with other commonly… CONTINUE READING
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