Super-SILAC mix for quantitative proteomics of human tumor tissue

  title={Super-SILAC mix for quantitative proteomics of human tumor tissue},
  author={Tamar Geiger and Juergen Cox and Pawel Ostasiewicz and Jacek R. Wisniewski and Matthias Mann},
  journal={Nature Methods},
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry–based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics. 
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A m er ic a, In c. A ll ri g h ts r es

  • J. Cox, Mann, M. Nat
  • 2008

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