Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction.

@article{Titlow2018SuperResolutionSM,
  title={Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction.},
  author={Joshua Scott Titlow and Lu Yang and Richard M. Parton and Ana Palanca and Ilan Davis},
  journal={Methods in molecular biology},
  year={2018},
  volume={1649},
  pages={
          163-175
        }
}
The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole mount Drosophila NMJ preparations to quantify primary transcription and count individual cytoplasmic mRNA molecules in specimens while maintaining ultrastructural preservation. The smFISH method is… CONTINUE READING