Sunflower (Helianthus annuus L.).

  title={Sunflower (Helianthus annuus L.).},
  author={Laura Mabel Radonic and Dalia Marcela Lewi and Nilda E L{\'o}pez and Horacio Esteban Hopp and Alejandro S. Escand{\'o}n and Marisa L{\'o}pez Bilbao},
  journal={Methods in molecular biology},
Sunflower (Helianthus annuus L.) is still considered as a recalcitrant species to in vitro culture and transformation in spite of the publication of different protocols. Here we describe a routine transformation system of this crop which requires mature HA89 genotype seeds and Agrobacterium tumefaciens EHA105 strain for gene delivery, being both easily available. Selection of transformed shoots depends on root development in kanamycin-selective media, instead of shoot color, avoiding selection… CONTINUE READING