Sumo-1 function is dispensable in normal mouse development.

  title={Sumo-1 function is dispensable in normal mouse development.},
  author={Fu-ping Zhang and Laura Mikkonen and Jorma Toppari and Jorma J. Palvimo and Irma Thesleff and Olli A. J{\"a}nne},
  journal={Molecular and cellular biology},
  volume={28 17},
To elucidate SUMO-1 functions in vivo, we targeted by homologous recombination the last three exons of the murine Sumo-1 gene. Sumo-1 mRNA abundance was reduced to one-half in heterozygotes and was undetectable in Sumo-1(-/-) mice, and SUMO-1-conjugated RanGAP1 was detectable in wild-type mouse embryo fibroblasts (MEFs) but not in Sumo-1(-/-) MEFs, indicating that gene targeting yielded Sumo-1-null mice. Sumo-1 mRNA is expressed in all tissues of wild-type mice, and its abundance is highest in… CONTINUE READING