Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides.

  title={Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides.},
  author={P. Feist and K. Danna},
  volume={20 15},
  • P. Feist, K. Danna
  • Published 1981
  • Chemistry, Medicine
  • Biochemistry
  • We have synthesized a new medium, sulfhydrylcellulose, for affinity chromatography of mercurated polynucleotides. It is the product of reaction between aminoethylcellulose and N-acetylhomocysteine thiolactone. Sulfhydrylcellulose carries up to 90 mumol of SH groups/g and is inexpensive, easy to prepare, and stable. Because it binds mercurated RNA specifically and reversibly and exhibits no size discrimination, sulfhydrylcellulose should have wide applications. 
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