Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides.

@article{Feist1981SulfhydrylcelluloseAN,
  title={Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides.},
  author={P. Feist and K. Danna},
  journal={Biochemistry},
  year={1981},
  volume={20 15},
  pages={
          4243-6
        }
}
  • P. Feist, K. Danna
  • Published 1981
  • Chemistry, Medicine
  • Biochemistry
  • We have synthesized a new medium, sulfhydrylcellulose, for affinity chromatography of mercurated polynucleotides. It is the product of reaction between aminoethylcellulose and N-acetylhomocysteine thiolactone. Sulfhydrylcellulose carries up to 90 mumol of SH groups/g and is inexpensive, easy to prepare, and stable. Because it binds mercurated RNA specifically and reversibly and exhibits no size discrimination, sulfhydrylcellulose should have wide applications. 
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    References

    SHOWING 1-9 OF 9 REFERENCES
    RNA aggregation during sulfhydryl-agarose chromatography of mercurated RNA.
    • 23
    Tissue sulfhydryl groups.
    • G. Ellman
    • Chemistry, Medicine
    • Archives of biochemistry and biophysics
    • 1959
    • 19,176
    RNA synthesis in isolated nuclei: the use of mercurated nucleotides.
    • 13
    Direct covalent mercuration of nucleotides and polynucleotides.
    • 141
    [31] Affinity chromatography
    • 1,246
    • PDF
    Transcription in vitro of immunoglobulin kappa light chain genes in isolated mouse myeloma nuclei and chromatin.
    • M. M. Smith, R. Huang
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1976
    • 46
    • PDF
    Strand orientation of simian virus 40 transcription in productively infected cells.
    • 28