Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model

@inproceedings{Hunter2016SulfationOT,
  title={Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model},
  author={Morag R Hunter and Natasha L Grimsey and Michelle R Glass},
  booktitle={Scientific reports},
  year={2016}
}
G protein-coupled receptors (GPCRs) are important therapeutic targets and therefore extensively studied. Like most transmembrane proteins, there has been considerable difficulty in developing reliable specific antibodies for them. To overcome this, epitope tags are often used to facilitate antibody recognition in studies on fundamental receptor signalling and trafficking. In our study of cannabinoid CB1/dopamine D2 interactions we sought to generate HEK293 cells expressing FLAG-tagged D2 for… CONTINUE READING

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