Sulfated fucan as support for antibiotic immobilization.

  title={Sulfated fucan as support for antibiotic immobilization.},
  author={Paulo M. Ara{\'u}jo and Givanildo Bezerra Oliveira and Carolina R. C{\'o}rdula and Edda Lisboa Leite and Luiz Bezerra de Carvalho and Marcia Alessandra Cavalaro Pereira da Silva},
  journal={Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas},
  volume={37 3},
Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25 masculine C and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a… CONTINUE READING