Sulfate Assimilation in C(4) Plants: Intercellular and Intracellular Location of ATP Sulfurylase, Cysteine Synthase, and Cystathionine beta-Lyase in Maize Leaves.

  title={Sulfate Assimilation in C(4) Plants: Intercellular and Intracellular Location of ATP Sulfurylase, Cysteine Synthase, and Cystathionine beta-Lyase in Maize Leaves.},
  author={James Nigel Burnell},
  journal={Plant physiology},
  volume={75 3},
  • J. Burnell
  • Published 1 July 1984
  • Biology, Medicine
  • Plant physiology
The activity of ATP sulfurylase, cysteine synthase, and cystathionine beta-lyase was measured in crude leaf extracts, bundle sheath strands, and mesophyll and bundle sheath chloroplasts to determine the location of sulfate assimilation of C(4) plant leaves. Almost all the ATP sulfurylase activity was located in the bundle sheath chloroplasts while cysteine synthase and cystathionine beta-lyase activity was located, in different proportions, in both chloroplast types.A new spectrophotometric… 
Cyst(e)ine is the transport metabolite of assimilated sulfur from bundle-sheath to mesophyll cells in maize leaves
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Nitrogen and Sulfur Metabolism in C 4 Plants
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Chapter 7 Nitrogen and Sulfur Metabolism in C4 Plants
C4 photosynthetic mechanism is based on a spatial separation of CO2 assimilating enzymes. The assimilation of two mineral nutrients, nitrogen and sulfur, is also localized in a cell-specific manner
Regulation of Sulfur Metabolism in Plants
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Regulation of sulfate assimilation in Arabidopsis and beyond.
It is demonstrated that investigations of other plant species will still be necessary to address specific questions of regulation of sulfur nutrition, as the role of Arabidopsis as a model plant will be further strengthened.
Localization of ATP Sulfurylase and O-Acetylserine(thiol)lyase in Spinach Leaves.
The intracellular compartmentation of ATP sulfurylase and O-acetylserine(thiol)lyase in spinach has been investigated by isolation of organelles and fractionation of protoplasts, and it is suggested that the plant cell may be unable to transport Cysteine between the different compartments, so that the cysteine required for protein synthesis must be synthesized in situ.
Are changes in sulfate assimilation pathway needed for evolution of C4 photosynthesis?
The information on sulfur assimilation provided by the recent transcriptomics data is assessed, their possible impact on understanding this interesting feature of plant sulfur metabolism is discussed and whether changes in sulfate assimilation are part of a general evolutionary trajectory toward C4 photosynthesis is investigated.
Redox regulation of ATP sulfurylase in microalgae.
It is found that the activity of ATPS-B from four different microorganisms was enhanced when reduced and decreased when oxidized, suggesting that life in the sea or freshwater may have driven the evolution of AT PS.
The role of 5′-adenylylsulfate reductase in controlling sulfate reduction in plants
The results provide additional evidence that APS reductase is a major control point for sulfate reduction in Z. mays, and the experimental basis for this hypothesis is reviewed.


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The activity of adenosine 5' triphosphate sulfurylase was determined in crabgrass mesophyll cells, bundle sheath strands, and whole leaf extracts, and a hypothesis is presented for the intercellular compartmentation of sulfur assimilation in relationship to NO(3) (-) and CO(2)Assimilation in leaves of C(4) plants.
Studies of l-Cysteine Biosynthetic Enzymes in Phaseolus vulgaris L.
Sulfur-deficient plants were grown and total extractable serine transacetylase activity was not affected by sulfur deficiency; in contrast, there was an increase in O-acetylserine sulfhydrylase activity under these conditions.
Initiation of Sulfate Activation: A Variation in C4 Photosynthesis Plants
In leaves of plants with C4 photosynthesis, sulfur assimilation is initiated in bundle sheath cells whereas carbon and nitrogen assimilation are initiated in mesophyll cells. The activation of
Intracellular localization of aspartate kinase and the enzymes of threonine and methionine biosynthesis in green leaves.
Threonine, like lysine, is synthesized only in the chloroplast, the last step in methionine biosynthesis occurs largely in the cytoplasm, and hence S-adenosylmethionine synthesis, is not found in any organelle fraction.
Cysteine synthase from rape leaves.
The purified preparation appeared to be homogeneous on Sephadex G-100 gel filtration and dodecylsulfate-polyacrylamide gel electrophoresis, showing a molecular weight of about 62,000 and suggesting that this enzyme was composed of two identical subunits.
  • D. Arnon
  • Chemistry, Medicine
    Plant physiology
  • 1949
Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
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Sulfur Amino Acids in Plants
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Chloroplast cysteine synthases of Trifolium repens and Pisum sativum
Cysteine synthase activity in leaf tissue of white clover and peas and peas was associated with chloroplasts and about 85 % of the O AS-dependent sulphide assimilated was recovered as cysteine.
Regulation of C4 photosynthesis: mechanism of activation and inactivation of extracted pyruvate, inorganic phosphate dikinase in relation to dark/light regulation.
It is concluded that interconversion of dithiol and disulfide forms of the enzyme is not critical for the dark/light regulation of pyruvate, Pi dikinase, and ATP may be a corequirment for ADP-dependent inactivation.