Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

@article{Hermosilla2015SuitableIV,
  title={Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription},
  author={Carlos Enrique Hermosilla and Ivonne Stamm and Christian Menge and Anja Taubert},
  journal={Parasitology Research},
  year={2015},
  volume={114},
  pages={3125-3136}
}
We here established a suitable in vitro cell culture system based on bovine colonic epithelial cells (BCEC) for the development of Eimeria bovis merozoites I and the characterization of early parasite-induced innate epithelial host cell reactions as gene transcription of proinflammatory molecules. Both primary and permanent BCEC (BCECprim and BCECperm) were suitable for E. bovis merozoite I invasion and subsequent development of meronts II leading to the release of viable merozoites II. E… CONTINUE READING
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