Suitability of cryopreservation for the long-term storage of rare and endangered plant species: a case history for Cosmos atrosanguineus.

@article{Wilkinson2003SuitabilityOC,
  title={Suitability of cryopreservation for the long-term storage of rare and endangered plant species: a case history for Cosmos atrosanguineus.},
  author={T Ross Wilkinson and Andrew C. Wetten and Chrissie Prychid and Michael F. Fay},
  journal={Annals of botany},
  year={2003},
  volume={91 1},
  pages={65-74}
}
The suitability of cryopreservation for the secure, long-term storage of the rare and endangered species Cosmos atrosanguineus was investigated. Using encapsulation/dehydration of shoot tips in alginate strips, survival rates of up to 100 % and shoot regeneration of up to 35 % were achieved. Light and electron microscopy studies indicated that cellular damage to some regions of the shoot tip during the freeze/thaw procedure was high, although cell survival in and around the meristematic region… CONTINUE READING