Sugars tied to the spot

  title={Sugars tied to the spot},
  author={Sabine L. Flitsch and Rein V. Ulijn},
The interactions of sugars and proteins underlie many biological processes, and cataloguing them is a daunting task. A technique for attaching sugars to microarrays offers a promising, high-throughput solution. 

Tools for decoding the structure-function relationships of biopolymers in nanotechnology and glycobiology

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2010.

Carbochips: a new energy for old biobuilders☆

Sialylglycan-modified Field Effect Transistor for Detection of Charged Lectin under Physiological Conditions

Determination of protein charges under physiological conditions by field effect transistors (FETs) is challenging because of the screening effect by solution counter ions. We showed that sugar-chai...

New Methods for the Generation of Carbohydrate Arrays on Glass Slides and Their Evaluation

Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino‐functionalized glass slides, preventing the necessity for the blocking of unreacted functional groups on the glass slide.

Solid-phase glycoconjugate synthesis : on-resin analysis with gel-phase ¹9F NMR spectroscopy

An efficient and versatile non-destructive method to analyze the progress of solid-phase glycoconjugate synthesis with gel-phase 19F NMR spectroscopy is described. The method relies on use of fluor

A lectin-based cell microarray approach to analyze the mammalian granulosa cell surface glycosylation profile

The described cell microarray method represents a simple and sensitive procedure to analyze cell surface glycome in mammalian cells and revealed that glycocalyx of both uGCs and cGCs contains N-glycans, sialic acid terminating glycans, N-acetylglucosamine and O- glycans.

Intact cell adhesion to glycan microarrays.

The described method is a robust approach to quantify selective cell adhesion using a wide variety of glycans and may contribute to the repertoire of tools for the study of glycomics.

Pattern recognition of monosaccharides via a virtual lectin array constructed by boronate affinity-based pH-featured encoding.

In this proof-of-concept work, without complicated synthesis or preparation, the proposed method was successful in mimicking lectin array and discriminating nine elementary monosaccharides found in nature, and it was a new way of encoding in expanding the applications of boronate affinity-based materials and methods in the field of biomimetics.

Recent Advances in the Synthesis and Biomedical Applications of Chain-End Functionalized Glycopolymers

This review summarizes recent advances in the synthesis and biomedical applications of chain-end functionalized glycopolymers.



Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions

We describe microarrays of oligosaccharides as neoglycolipids and their robust display on nitrocellulose. The arrays are sourced from glycoproteins, glycolipids, proteoglycans, polysaccharides, whole

Synthesis of sugar arrays in microtiter plate.

1,3-Dipolar cycloadditions between azides and alkynes were exploited to attach oligosaccharides to a C(14) hydrocarbon chain that noncovalently binds to the microtiter well surface to achieve biotransformation of beta-galactosyllipid 5.

Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells

The development of a carbohydrate-based microarray to extend the scope of biomedical research on carbohydrate-mediated molecular recognition and anti-infection responses is described and the potential of carbohydrate microarrays is demonstrated by the discovery of previously undescribed cellular markers, Dex-Ids.

Nature Biotechnol

  • Nature Biotechnol
  • 2002

Chem. Biol

  • Chem. Biol
  • 2002

Nature Biotechnol

  • Nature Biotechnol
  • 2002

J. Am. Chem. Soc

  • J. Am. Chem. Soc
  • 2002