Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis

@article{Biswal2018SugarRA,
  title={Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis},
  author={Ajaya Kumar Biswal and Melani A Atmodjo and Mi Li and Holly L. Baxter and Chang Geun Yoo and Yunqiao Pu and Yi-Ching Lee and Mitra Mazarei and Ian M Black and Ji-Yi Zhang and Hema Ramanna and Adam L. Bray and Zachary R. King and Peter R. Lafayette and Sivakumar Pattathil and Bryon S Donohoe and Sushree S. Mohanty and David Ryno and Kelsey Yee and Olivia A Thompson and Miguel Rodriguez and Alexandru Dumitrache and Jace M Natzke and Kim Winkeler and Cassandra Collins and Xiaohan Yang and Li Tan and Robert W. Sykes and Erica L. Gjersing and Angela L. Ziebell and Geoffrey B. Turner and Stephen R Decker and Michael G Hahn and Brian H. Davison and Michael K. Udvardi and Jonathan R. Mielenz and Mark F Davis and Richard S Nelson and Wayne Parrott and Arthur J Ragauskas and C. Neal Stewart and Debra Mohnen},
  journal={Nature Biotechnology},
  year={2018},
  volume={36},
  pages={249-257}
}
Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. We engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to… CONTINUE READING
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Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

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