Sugar binding to Na+/glucose cotransporters is determined by the carboxyl-terminal half of the protein.

@article{PanayotovaHeiermann1996SugarBT,
  title={Sugar binding to Na+/glucose cotransporters is determined by the carboxyl-terminal half of the protein.},
  author={M Panayotova-Heiermann and Donald D. F. Loo and Cheuk Ting Kong and Julia E. Lever and Ernest M Wright},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 17},
  pages={10029-34}
}
d-Glucose is absorbed across the proximal tubule of the kidney by two Na+/glucose cotransporters (SGLT1 and SGLT2). The low affinity SGLT2 is expressed in the S1 and S2 segments, has a Na+:glucose coupling ratio of 1, a K0.5 for sugar of approximately 2 mM, and a K0.5 for Na+ of approximately 1 mM. The high affinity SGLT1, found in the S3 segment, has a coupling ratio of 2, and K0.5 for sugar and Na+ of approximately 0.2 and 5 mM, respectively. We have constructed a chimeric protein consisting… CONTINUE READING