Sucrose uptake into vacuoles of sugarcane suspension cells

  title={Sucrose uptake into vacuoles of sugarcane suspension cells},
  author={Joachim Preisser and Ewald Komor},
Uptake of sucrose into vacuoles of suspension cells of Saccharum sp. (sugarcane) was investigated using a vacuole-isolation method based on osmotic- and pH-dependent lysis of protoplasts. Vacuoles took up sucrose at high rates without the influence of tonoplast energization on sucrose transport. Neither addition of ATP or pyrophosphate nor dissipation of the membrane potential or the pH gradient by ionophores changed uptake rates appreciably. Generation of an ATP-dependent pH gradient across… CONTINUE READING


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