Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum

@article{MllerSchweinitzer2004SucrosePT,
  title={Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum},
  author={Else M{\"u}ller‐Schweinitzer and P Ellis},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2004},
  volume={345},
  pages={594-597}
}
SummaryThe cryoprotective effect of sucrose has been investigated using 3 different vascular smooth muscle preparations, namely canine saphenous veins and arteries and porcine circumflex coronary arteries following storage in liquid nitrogen (at −196°C). Contractile responses to noradrenaline, 5-HT, prostaglandin F2α and KCl and relaxant responses to substance P and 5-HT were determined on fresh tissues and after cryostorage in fetal calf serum (FCS) containing either 1.8 M dimethyl sulfoxide… 

Cryopreservation of vascular tissues

Evidence from pharmacological studies suggests that cryopreservation induces tissue specific changes in transmembrane signaling and the mechanisms coupling intracellular calcium release, sensitivity and calcium entry into the smooth muscle cells.

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

The use of TiProtec or modifications thereof as base solution for cryopreservation greatly improved cryop Reservation results for endothelial monolayers in terms of survival and of monolayer and mitochondrial integrity.

Optoelectronic method for increasing the sensibility of mass spectrometer in the case of the determination of papaverine

Papaverine is an opiate alkaloid used in the primary treatment of visceral spasms, cardiac and cerebral vasospasms, and occasionally for the treatment of erectile dysfunction, but its use for the latest recommendations should be regarded with caution.

Opium Poppy: Genetic Upgradation Through Intervention of Plant Breeding Techniques

The whole plant parts of opium poppy are valuable in terms of food, medicine, vegetable and as brew‐ brewed drugs.

Intraoperative Somatosensory and Motor Evoked Potential Recording and Electroencephalographic Monitoring Usefulness During Aneurysm Surgery

This chapter reports the experience in using neurophysiopathological monitoring during aneurysm surgery with particular reference at the evoked potential and EEG changes in response to surgical and anaesthetic variables.

References

SHOWING 1-10 OF 20 REFERENCES

Functional studies on human veins after storage at − 190°C

It is suggested that cryopreservation is a useful technique for storing human veins for pharmacological studies and that after thawing of frozen stored veins the contractile force development was unchanged, and the evidence suggested that the monoamine oxidase activity was unimpaired.

Maintenance of functional activity of human pulmonary arteries after cryopreservation

The evidence suggests that after cryopreservation of human intrapulmonary arteries at −190°C, mechanisms of both contraction and relaxation are well‐maintained.

Cryopreservation of innervation, endothelial and vascular smooth muscle function of a rabbit muscular and resistance artery.

The extent of preservation of endothelial, neurogenic and vascular function following frozen storage was studied using the rabbit central ear artery and a resistance artery branch, suggesting that adrenergic nerve function is well preserved.

Pharmacological studies on frozen stored canine saphenous veins and basilar arteries

It is concluded that freezing isolated blood vessels may be considered an effective means of preserving and storing vascular tissues for pharmacological investigations.

The Preservation of Functional Activity of Smooth Muscle and Endothelium in Pig Coronary Arteries after Storage at −190°C

The evidence suggests that after cryopreservation of pig coronary arteries at −190° C mechanisms of relaxation, in particular those which are endothelium‐dependent, are well maintained.

Effects of calcium channel blockade in canine saphenous veins after storage at −190°C

It is suggested that cryopreservation is a useful technique for storing venous smooth muscle for pharmacological studies and a good preservation of both contractile responsiveness and 45Ca2+ uptake mechanisms after storage at −190°C is suggested.

Mechanism of cryoinjury in biological systems.