Suckling mice were used to detect infectious dengue-2 viruses by intracerebral injection of the full-length RNA transcript.

@article{Lee2005SucklingMW,
  title={Suckling mice were used to detect infectious dengue-2 viruses by intracerebral injection of the full-length RNA transcript.},
  author={Ying-Ray Lee and K Huang and Huan-Yao Lei and Shunhua Chen and Y S Lin and Trai-Ming Yeh and Hsiao-Sheng Liu},
  journal={Intervirology},
  year={2005},
  volume={48 2-3},
  pages={161-6}
}
OBJECTIVE In this study we established a mouse brain injection system to detect infectious dengue-2 virus produced from the full-length RNA transcripts. METHODS In vitro transcription was used to synthesize full-length dengue-2 virus RNA from the plasmid pRS424FLDEN2NGC, which was intracerebrally injected into the 6-day-old suckling mice (ICR strains). Engineered dengue-2 viruses were detected in the brain sections using immunohistochemistry staining. RT-PCR followed by restriction… CONTINUE READING