Successful production of pseudotyped rAAV vectors using a modified baculovirus expression system.

@article{Kohlbrenner2005SuccessfulPO,
  title={Successful production of pseudotyped rAAV vectors using a modified baculovirus expression system.},
  author={Erik Kohlbrenner and George V. Aslanidi and Kevin Nash and Stanislav Shklyaev and Martha L Campbell-Thompson and Barry J. Byrne and Richard O. Snyder and Nicholas Muzyczka and Kenneth H. Warrington and Sergei Zolotukhin},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2005},
  volume={12 6},
  pages={
          1217-25
        }
}
  • Erik Kohlbrenner, George V. Aslanidi, +7 authors Sergei Zolotukhin
  • Published in
    Molecular therapy : the…
    2005
  • Biology, Medicine
  • Scalable production of rAAV vectors remains a major obstacle to the clinical application of this prototypical gene therapy vector. A recently developed baculovirus-based production protocol (M. Urabe et al., 2002, Hum. Gene Ther. 13, 1935-1943) found limited applications due to the system's design. Here we report a detailed analysis of the stability of the original baculovirus system components BacRep, BacVP, and transgene cassette-containing BacGFP. All of the baculovirus helpers analyzed were… CONTINUE READING

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