Successful isolation and long-term establishment of a cell line with stem cell-like features from an anaplastic medulloblastoma.

@article{Raso2008SuccessfulIA,
  title={Successful isolation and long-term establishment of a cell line with stem cell-like features from an anaplastic medulloblastoma.},
  author={Alessandro Raso and Francesca Negri and Andrea Gregorio and Paolo Nozza and Samantha Mascelli and Patrizia De Marco and Elisa Merello and Claudia Milanaccio and Marcello Ravegnani and A. Cama and Maria Luisa Garr{\'e} and Valeria Capra},
  journal={Neuropathology and applied neurobiology},
  year={2008},
  volume={34 3},
  pages={306-15}
}
AIMS Herein we report on the successful isolation and establishment of a novel, long-term, primary, neurosphere-like cell line called 1603-MED from a 5-year-old boy affected by a highly aggressive anaplastic medulloblastoma. METHODS Elaboration of the new protocol for neurosphere assay is extensively discussed, together with a complete immuno-histochemical and cytogenetic characterization of 1603-MED. RESULTS Clinical course and histopathology are briefly discussed. The 1603-MED possesses a… CONTINUE READING

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