Successful induction of sclerostin in human-derived fibroblasts by 4 transcription factors and its regulation by parathyroid hormone, hypoxia, and prostaglandin E2.

@article{Fujiwara2016SuccessfulIO,
  title={Successful induction of sclerostin in human-derived fibroblasts by 4 transcription factors and its regulation by parathyroid hormone, hypoxia, and prostaglandin E2.},
  author={Makoto T Fujiwara and Takuo Kubota and Wei Wang and Yasuhisa Ohata and Kohji Miura and Taichi Kitaoka and Daisuke Okuzaki and Noriyuki Namba and Toshimi Michigami and Yasuji Kitabatake and Keiichi Ozono},
  journal={Bone},
  year={2016},
  volume={85},
  pages={91-8}
}
Sclerostin, coded by SOST, is a secretory protein that is specifically expressed in osteocytes and suppresses osteogenesis by inhibiting WNT signaling. The regulatory mechanism underlying SOST expression remains unclear mainly due to the absence of an adequate human cell model. Thus, we herein attempted to establish a cell model of human dermal fibroblasts in order to investigate the functions of sclerostin. We selected 20 candidate transcription factors (TFs) that induce SOST expression by… CONTINUE READING