Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker

@article{Min2006SuccessfulGT,
  title={Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker},
  author={Byung-Whan Min and Yi-Nam Cho and Min-Jung Song and Tae-Kyung Noh and B Kim and Won-Ki Chae and Young-Su Park and Yang-Do Choi and Chee-Hark Harn},
  journal={Plant Cell Reports},
  year={2006},
  volume={26},
  pages={337-344}
}
A mannose selection system was adapted for use in the Agrobacterium-mediated transformation of Chinese cabbage. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate. Hypocotyl explants from 4–5-day-old seedlings of Chinese cabbage inbred lines were pre-cultured for 2–3 days and then infected with Agrobacterium. Two genes (l-guluno-γ-lactone oxidase, GLOase, and jasmonic methyl transferase, JMT) were transformed… CONTINUE READING

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