Successful analysis of AFLPs from non-lethally sampled wing tissues in butterflies

  title={Successful analysis of AFLPs from non-lethally sampled wing tissues in butterflies},
  author={Nusha Keyghobadi and Lindsay A. Crawford and Sheri A. Maxwell},
  journal={Conservation Genetics},
We demonstrate the successful generation and analysis of amplified fragment length polymorphism (AFLP) profiles from small samples of wing tissue in two butterfly species. With slight modifications of commercial DNA extraction and AFLP kit protocols, we produced highly repeatable AFLP profiles from these non-destructive tissue samples. Error rates were comparable to those previously reported for AFLPs generated from lethally obtained thoracic tissue. Furthermore, AFLP profiles obtained from… CONTINUE READING
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ena helle (Lepidoptera: Lycaenidae)

  • N Keyghobadi, KP Unger, JD Weintraub, DM Fonseca
  • Eur J Entomol
  • 2006
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