Successful Isolation of Infectious and High Titer Human Monocyte-Derived HIV-1 from Two Subjects with Discontinued Therapy

@inproceedings{Wang2013SuccessfulIO,
  title={Successful Isolation of Infectious and High Titer Human Monocyte-Derived HIV-1 from Two Subjects with Discontinued Therapy},
  author={Tong Wang and Younong Xu and Haiying Zhu and Thomas Andrus and Sergei B. Ivanov and Charlotte Pan and Jazel S. Dolores and Gregory C. Dann and Michael X. Zhou and Dominic Forte and Zihuan Yang and Sarah E Holte and Lawrence Corey and Tuofu Zhu},
  booktitle={PloS one},
  year={2013}
}
BACKGROUND HIV-1 DNA in blood monocytes is considered a viral source of various HIV-1 infected tissue macrophages, which is also known as "Trojan horse" hypothesis. However, whether these DNA can produce virions has been an open question for years, due to the inability of isolating high titer and infectious HIV-1 directly from monocytes. RESULTS In this study, we demonstrated successful isolation of two strains of M-HIV-1 (1690 M and 1175 M) from two out of four study subjects, together with… CONTINUE READING