Subunit stoichiometry of a core conduction element in a cloned epithelial amiloride-sensitive Na+ channel.

@article{Berdiev1998SubunitSO,
  title={Subunit stoichiometry of a core conduction element in a cloned epithelial amiloride-sensitive Na+ channel.},
  author={Bakhrom K Berdiev and Katherine H Karlson and Biljana Jovov and P J Ripoll and Roger Stewart Morris and Dominique Loffing-Cueni and P. K. Raju Chetan S. Sankar Gerald Halpin Glennelle Halpin and Bruce A. Stanton and Thomas R Kleyman and Iskander I. Ismailov},
  journal={Biophysical journal},
  year={1998},
  volume={75 5},
  pages={2292-301}
}
The molecular composition of a core conduction element formed by the alpha-subunit of cloned epithelial Na+ channels (ENaC) was studied in planar lipid bilayers. Two pairs of in vitro translated proteins were employed in combinatorial experiments: 1) wild-type (WT) and an N-terminally truncated alphaDeltaN-rENaC that displays accelerated kinetics (tauo = 32 +/- 13 ms, tauc = 42 +/- 11 ms), as compared with the WT channel (tauc1 = 18 +/- 8 ms, tauc2 = 252 +/- 31 ms, and tauo = 157 +/- 43 ms… CONTINUE READING