Subunit organization of PSI particles from brown algae and diatoms: polypeptide and pigment analysis

  title={Subunit organization of PSI particles from brown algae and diatoms: polypeptide and pigment analysis},
  author={Claire Berkaloff and Lise Caron and Bernard Rousseau},
  journal={Photosynthesis Research},
P700 enriched fractions were isolated from two brown algae and one diatom using sucrose density centrifugation after digitinin solubilization. They had a Chl a/P700 ratio of about 250 to 375 according to the species, they were enriched in long-wavelength absorbing Chl a and exhibited a fluorescence emission maximum at 77 K near 720 nm. They all presented a major polypeptide component at 66±2 kDa, but their polypeptide composition was rather complex and somewhat different from one species to… 

Light-Harvesting Systems in Chlorophyll c-Containing Algae

All the light-harvesting proteins seem to be encoded by multigene families, and for diatoms and brown algae, many genes have been sequenced, which will allow the role of individual gene products in determining the adaptive responses to environmental variation to be appraised.

Utilization of light by fucoxanthin–chlorophyll-binding protein in a marine centric diatom, Chaetoceros gracilis

Fluorescence excitation spectra analyses indicated that light harvesting, rather than photosystem protection, is the major function of the purified FCP complex, which is associated with more than 60 % of total cellular Chl a.

Light-harvesting proteins of diatoms: Their relationship to the chlorophyll a/b binding proteins of higher plants and their mode of transport into plastids

Members of a gene family encoding polypeptide constituents of the fucoxanthin, chlorophyll a/c protein complex, a light-harvesting complex associated with photosystem II of diatoms and brown algae are cloned and characterized.

Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis.

Spectroscopic and molecular characterization of the oligomeric antenna of the diatom Phaeodactylum tricornutum.

The spectroscopic characteristics and pigment stoichiometries of the FCPo complex were comparable to FCP complexes that were isolated after solubilization with higher detergent per chlorophyll ratios, indicating that these complexes may represent the native form of the diatom antenna system in the thylakoid membrane of P. tricornutum.

Structurally flexible macro-organization of the pigment–protein complexes of the diatom Phaeodactylum tricornutum

In intact cells the main CD band at (+)698 nm appeared to be associated with the multilamellar organization of the thylakoid membranes, which was sensitive to the osmotic pressure and was selectively diminished at elevated temperatures and was capable of undergoing light-induced reversible changes.

Assembly of the photosynthetic apparatus in embryos from Fucus serratus L

Spectral modifications revealing the progressive integration of Chl a and Chl c in the photosynthetic units are described and room temperature variable fluorescence was first observed 30 h after fertilization indicating a functional Photosystem II electron transfer at this developmental stage.

The role of the LHCX light-harvesting complex protein family in diatom photoprotection

This work highlighted the functional diversification and the importance of the LHCX protein family in the fine-tuning of light harvesting and photoprotection capacity, possibly contributing to explain diatoms success in their highly fluctuating environment.

Structure of PSI, PSII and antennae complexes from yellow-green alga Xanthonema debile

Structural maps of photosystem I, photosystem II and light harvesting antenna complexes isolated from a soil chromophytic alga Xanthonema debile revealed that the overall structure of Xanthophytes’ PSI and PSII complexes is similar to that known from higher plants or algae.



A diatom light-harvesting pigment-protein complex : purification and characterization.

A light-harvesting pigment-protein complex was isolated from the diatom Phaeodactylum tricornutum using the zwitterionic detergent CHAPS and it appears that this complex is common to other diatom species.

Chlorophyll-protein complexes of barley photosystem I.

Reconstitution studies demonstrated that excitation energy transfer from LHCII to PSI requires the presence of LHCI-680, and it is proposed that, in PSI, the following linear excitationEnergy transfer sequence occurs.

Chlorophyll proteins of photosystem I.

Data are presented which suggest the existence of a light-harvesting pigment-protein complex which is functionally and structurally associated with photosystem I (PSI) reaction centers. These

Polypeptides of a Light-Harvesting Complex of the Diatom Phaeodactylum tricornutum Are Synthesized in the Cytoplasm of the Cell as Precursors.

Investigation of a light-harvesting fucoxanthin-chlorophyll a/c-protein complex isolated from the diatom Phaeodactylum tricornutum by detergent extraction of thylakoid membranes coupled with sucrose density gradient centrifugation indicates that the polypeptide components are synthesized as precursors 3,000 to 5,000 daltons larger than the maturepolypeptides.