Subunit dynamics in Escherichia coli preprotein translocase.

  title={Subunit dynamics in Escherichia coli preprotein translocase.},
  author={J{\'u}lio C{\'e}sar Joly and Marilyn Rice Leonard and William Wickner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={91 11},
SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase. To measure the in vivo association of these polypeptides and assay possible exchange, plasmid-borne secY and secE genes were placed under control of the ara regulon and fused to DNA encoding the influenza hemagglutinin epitope. Cells were incubated with [35S]methionine, grown for a "chase" period, and then induced with arabinose to express epitope-tagged, nonradioactive SecY and… CONTINUE READING

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