Subtypes of odorant-binding proteins--heterologous expression and ligand binding.

  title={Subtypes of odorant-binding proteins--heterologous expression and ligand binding.},
  author={Dietrich L{\"o}bel and Stefano Marchese and J{\"u}rgen Krieger and Paolo Pelosi and Heinz Breer},
  journal={European journal of biochemistry},
  volume={254 2},
Odorant-binding proteins (OBP) in the mucus of the olfactory epithelium are thought to transfer the hydrophobic odorous compounds through the aqueous barrier towards the chemo-sensory cells. To evaluate their binding properties, two distinct OBP subtypes of the rat were expressed as N-terminal His-tagged fusion proteins in Escherichia coli, thus allowing an efficient purification. Based on gel chromatography and CD spectroscopy analysis the recombinant OBP subtypes seem to share several… CONTINUE READING


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