Subtilisin removes the surface layer of the phage fd coat.

  title={Subtilisin removes the surface layer of the phage fd coat.},
  author={P Schwind and Haiman Kramer and A Kremser and U Ramsberger and Ihab Rasched},
  journal={European journal of biochemistry},
  volume={210 2},
The major coat protein of native filamentous phage fd is vulnerable to digestion by subtilisin, but not by any of a number of other proteolytic enzymes. Degradation by the non-specific protease subtilisin occurs at specific sites in the N-terminal portion of g8p. The N-terminal part of the protein is considered to be the outer layer of a two-layered coat. Thus, subtilisin treatment results in a monolayered phage particle. These particles possess the morphology and stability of native phage fd… CONTINUE READING

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