Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.

@article{Thomasy2012SubstratumSA,
  title={Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.},
  author={Sara M. Thomasy and Joshua A. Wood and Philip H Kass and Christopher John Murphy and Paul Russell},
  journal={Investigative ophthalmology & visual science},
  year={2012},
  volume={53 2},
  pages={952-8}
}
PURPOSE To determine the impact of substratum stiffness and latrunculin-B (Lat-B), on the expression of several matrix proteins that are associated with glaucoma. METHODS Human trabecular meshwork (HTM) cells were cultured on hydrogels possessing stiffness values mimicking those found in normal (5 kPa) and glaucomatous meshworks (75 kPa), or tissue… CONTINUE READING