Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.

@article{Thomasy2012SubstratumSA,
  title={Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.},
  author={Sara M. Thomasy and Joshua A. Wood and Philip H Kass and Christopher John Murphy and Paul Russell},
  journal={Investigative ophthalmology & visual science},
  year={2012},
  volume={53 2},
  pages={952-8}
}
PURPOSE To determine the impact of substratum stiffness and latrunculin-B (Lat-B), on the expression of several matrix proteins that are associated with glaucoma. METHODS Human trabecular meshwork (HTM) cells were cultured on hydrogels possessing stiffness values mimicking those found in normal (5 kPa) and glaucomatous meshworks (75 kPa), or tissue culture polystyrene (TCP; >1 GPa). Cells were treated with 2.0 μM Lat-B in dimethyl sulfoxide (DMSO) or DMSO alone. RT-PCR was used to determine… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 51 references

Functional morphology of the trabecular meshwork in primate eyes.

Progress in retinal and eye research • 1999
View 5 Excerpts
Highly Influenced

Electron microscopic studies on the trabecular meshwork in glaucoma simplex

Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie • 1972
View 5 Excerpts
Highly Influenced

Experimental aqueous perfusion in enucleated human eyes.

Archives of ophthalmology • 1963
View 5 Excerpts
Highly Influenced

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