Substrate stiffness influences TGF-β1-induced differentiation of bronchial fibroblasts into myofibroblasts in airway remodeling.

@article{Shi2013SubstrateSI,
  title={Substrate stiffness influences TGF-β1-induced differentiation of bronchial fibroblasts into myofibroblasts in airway remodeling.},
  author={Yanling Shi and Yuhui Dong and Yiyuan Duan and Xuemei Jiang and Cheng Chen and Linhong Deng},
  journal={Molecular medicine reports},
  year={2013},
  volume={7 2},
  pages={419-24}
}
Chronic inflammation and remodeling of the bronchial wall are basic hallmarks of asthma. During the process of bronchial wall remodeling, inflammatory factors, such as transforming growth factor-β1 (TGF-β1), are known to induce the differentiation of fibroblasts into myofibroblasts, which leads to excessive synthesis and secretion of extracellular matrix (ECM) proteins, thus thickening and stiffening the basement membrane. However, it has not been thoroughly studied whether or not substrate… CONTINUE READING

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During the process of bronchial wall remodeling , inflammatory factors , such as transforming growth factor-β1 ( TGF-β1 ) , are known to induce the differentiation of fibroblasts into myofibroblasts , which leads to excessive synthesis and secretion of extracellular matrix ( ECM ) proteins , thus thickening and stiffening the basement membrane .
During the process of bronchial wall remodeling , inflammatory factors , such as transforming growth factor-β1 ( TGF-β1 ) , are known to induce the differentiation of fibroblasts into myofibroblasts , which leads to excessive synthesis and secretion of extracellular matrix ( ECM ) proteins , thus thickening and stiffening the basement membrane .
During the process of bronchial wall remodeling , inflammatory factors , such as transforming growth factor-β1 ( TGF-β1 ) , are known to induce the differentiation of fibroblasts into myofibroblasts , which leads to excessive synthesis and secretion of extracellular matrix ( ECM ) proteins , thus thickening and stiffening the basement membrane .
During the process of bronchial wall remodeling , inflammatory factors , such as transforming growth factor-β1 ( TGF-β1 ) , are known to induce the differentiation of fibroblasts into myofibroblasts , which leads to excessive synthesis and secretion of extracellular matrix ( ECM ) proteins , thus thickening and stiffening the basement membrane .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
Myofibroblast differentiation was examined using semi - quantitative RT - PCR for the expression of α-smooth muscle actin ( α-SMA ) mRNA and collagen I mRNA , the enzyme - linked immunosorbent assay method was used to assess the expression of collagen I protein and western blotting to assess the expression of α-SMA protein .
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