Substrate specificity of the protein tyrosine phosphatases.

  title={Substrate specificity of the protein tyrosine phosphatases.},
  author={Zhihong Zhang and A M Thieme-Sefler and Derrick Maclean and Dennis J McNamara and Ellen M Dobrusin and Tomi K. Sawyer and Jack E. Dixon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 10},
The substrate specificity of a recombinant protein tyrosine phosphatase (PTPase) was probed using synthetic phosphotyrosine-containing peptides corresponding to several of the autophosphorylation sites in epidermal growth factor receptor (EGFR). The peptide corresponding to the autophosphorylation site, EGFR988-998, was chosen for further study due to its favorable kinetic constants. The contribution of individual amino acid side chains to the binding and catalysis was ascertained utilizing a… CONTINUE READING