Substrate specificity of the paraffin hydroxylase ofPseudomonas aeruginosa

@article{RavenswaayClaasen2005SubstrateSO,
  title={Substrate specificity of the paraffin hydroxylase ofPseudomonas aeruginosa},
  author={J. C. Ravenswaay Claasen and A Linden},
  journal={Antonie van Leeuwenhoek},
  year={2005},
  volume={37},
  pages={339-352}
}
The paraffin hydroxylating enzyme system isolated fromPseudomonas aeruginosa (strain 473) grown onn-heptane has been studied with respect to its substrate specificity. It has been found that cell-free extracts of this organism can hydroxylate a wide variety of hydrocarbons. In order to function as substrates, molecules should be able to assume a more or less planar conformation. The compounds used in this study can be divided into three classes: (a) alkanes, (b) alkylbenzenes and (c) (alkyl… Expand
13 Citations
Diversity, Function, and Biocatalytic Applications of Alkane Oxygenases
This chapter focuses on the aerobic degradation of hydrocarbons, with an emphasis on alkanes, and the applications of enzymes involved in these processes for biocatalysis. Yeasts and fungi are oftenExpand
Microbial transformation of tetralin.
Biocatalytic oxidation of cyclic hydrocarbons has many potential applications in the production of fine chemicals. Especially regioselective hydroxylation of aromatics and the stereospecificExpand
Genetics of alkane oxidation byPseudomonas oleovorans
TLDR
The genetics and enzymology of alkane metabolism have been investigated in depth for Pseudomonas oleovorans, which is able to oxidize C5-C12 n-alkanes by virtue of two gene regions, localized on the OCT-plasmid. Expand
Investigation of the prevalence and catalytic activity of rubredoxin-fused alkane monooxygenases (AlkBs).
TLDR
Results are consistent with this mutation causing a structural change impacting substrate positioning, as the products of V91W-mutant-catalyzed oxidation of alkenes had a higher aldehyde-to-epoxide ratio than the products formed in the presence of the wild type protein. Expand
Initial steps in the degradation of n-alkane-1-sulphonates by Pseudomonas
TLDR
The primary reaction in the degradation of n-alkane-1-sulfonates by Pseudomonas is the hydroxylation of the carbon atom bearing the sulfonate group, which formed easily hydrolyses to give the corresponding aldehyde and bisulfite. Expand
Alkane hydroxylase systems in Pseudomonas aeruginosa strains able to grow on n-octane
Summary Pseudomonas strains able to grow on n-octane were selected from different strain collections, and compared with P. oleovorans GPo1 with respect to the genetics of alkane metabolism in theseExpand
Isolierung, Substratassimilation und einige Produkte alkanabbauender Schimmelpilze
Summary1.Six strains of alkane assimilating molds (belonging to the order of the Mucorales and Moniliales) were isolated from 120 different samples of soil, taken from the parks of Muenster. The soilExpand
Oxidation of gasoline oxygenates by closely related non-haem-iron alkane hydroxylases in Pseudomonas mendocina KR1 and other n-octane-utilizing Pseudomonas strains.
TLDR
Results indicate ether oxygenate-degrading activity in alkane-utilizing pseudomonads is consistently observed with close homologues of the GPo1 non-haem-iron alkane hydroxylases but is otherwise not a consistent catalytic feature of these diverse enzymes. Expand
Alkane Degradation by Pseudomonads
Alkanes are highly reduced hydrocarbon compounds that constitute about 20–50% of crude oil. They are produced by geochemical processes from decaying plant and algal material, end up in theExpand
Bacterial degradation of cyclohexane participation of a co-oxidation reaction
AbstractComplete biodegradation of cyclohexane was demonstrated to occur in a system containing two strains of Pseudomonad bacteria, viz an n-alkane oxidizer and a microorganism utilizingExpand
...
1
2
...

References

SHOWING 1-10 OF 34 REFERENCES
Occurrence of inducible and NAD(P)-independent primary alcohol dehydrogenases in an alkane-oxidizingPseudomonas
TLDR
Pseudomonas aeruginosa constitutively contains a soluble NADP-linked dehydrogenase active towards primary alcohols and at least two NAD(P)-independent primary alcohol dehydrogenases can be induced by growing this strain onPrimary alcohols,α,ω-diols orn-alkanes. Expand
Paraffin Oxidation in Pseudomonas aeruginosa I. Induction of Paraffin Oxidation
TLDR
The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated and a number of compounds which did not sustain growth were found to be suitable substitutes for paraffins as an inducer. Expand
Bacterial oxidation of cycloparaffinic hydrocarbons
TLDR
A series of 10 branched-chain alkanes and 4 cycloalkanes were employed individually as elective culture substrates for bacteria in soil and metabolic products identified are consistent with the formation of a cyclomonoalcohol as the immediate precursor of the ketone. Expand
A soluble cytochrome P-450 functional in methylene hydroxylation.
TLDR
The coupling of the hydroxylase to the reduced diphosphopyridine nucleotide dehydrogenase (reductase), and the putidaredoxin is demonstrated, and substrate is shown to be required for the over-all reaction. Expand
n-Alkane oxidation by aPseudomonas
Summary1.APseudomonas oxidizingn-alkanes adaptively was used in experiments on hexane and heptane degradation.2.The R.Q. of heptane respiration by resting heptane-adapted cells was found to be 0.62.Expand
Enzymatic ω-Oxidation II. FUNCTION OF RUBREDOXIN AS THE ELECTRON CARRIER IN ω-HYDROXYLATION
Abstract Molecular oxygen and reduced diphosphopyridine nucleotide are required for the hydroxylation of octane, yielding n-octanol, in a soluble enzyme system from Pseudomonas oleovorans previouslyExpand
Cytochrome P-450 involvement in the oxidation of n-octane b cell-free extracts of Corynebacterium sp. strain 7E1C.
TLDR
It has been demonstrated that cell-free extracts of Corynebacterium sp. Expand
Oxidation of alkyl-substituted cyclic hydrocarbons by a Nocardia during growth on n-alkanes.
TLDR
The specificity of microbial oxidations is demonstrated by the conversion of p-isopropyltoluene (p-cymene) to p- isopropylbenzoic acid in n-alkane, growth-supported nocardia cultures. Expand
Enzymatic ω-Oxidation I. ELECTRON CARRIERS IN FATTY ACID AND HYDROCARBON HYDROXYLATION
Abstract The separation of the soluble ω-hydroxylation system of Pseudomonas oleovorans into three components is reported in this paper. They have been identified as rubredoxin (a red proteinExpand
Studies on the utilization of hydrocarbons by microorganisms,1.
During the course of an investigation of the microbial assimilation of aromatic hydrocarbons, several strains were found to produce a large amount of cumic acid from p-cymene.Five strains, S449B1,Expand
...
1
2
3
4
...