Substrate specificity of alpha-1,6-mannosyltransferase that initiates N-linked mannose outer chain elongation in Saccharomyces cerevisiae.

@article{Nakayama1997SubstrateSO,
  title={Substrate specificity of alpha-1,6-mannosyltransferase that initiates N-linked mannose outer chain elongation in Saccharomyces cerevisiae.},
  author={Keiichi I Nakayama and Y. Nakanishi-Shindo and Aki Tanaka and Y Haga-Toda and Y. Jigami},
  journal={FEBS letters},
  year={1997},
  volume={412 3},
  pages={547-50}
}
Yeast Saccharomyces cerevisiae OCH1 gene encodes the mannosyltransferase that is essential for the outer chain elongation of N-linked oligosaccharides. Mannosyltransferase activity of OCH1 gene product (Och1p) was measured on HPLC by using pyridylaminated Man8GlcNAc2 (Man8GlcNAc2-PA) as an acceptor and the reaction product was observed at the retention time corresponding to Man9GlcNAc2-PA. 1H-NMR and fast atom bombardment mass spectrometry (FAB-MS) fragmentation analysis of Man9GlcNAc2-PA… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Similar Papers

Loading similar papers…