Substrate specificity of recombinant human immunodeficiency virus integrase protein.

@article{Lafemina1991SubstrateSO,
  title={Substrate specificity of recombinant human immunodeficiency virus integrase protein.},
  author={Robert L Lafemina and Peggy Callahan and Michael G. Cordingley},
  journal={Journal of virology},
  year={1991},
  volume={65 10},
  pages={5624-30}
}
Recombinant human immunodeficiency virus type 1 (HIV-1) integrase (IN) produced in Escherichia coli efficiently cleaves two nucleotides from the 3' end of synthetic oligonucleotide substrates which mimic the termini of HIV-1 proviral DNA. Efficient cleavage was restricted to HIV-1 substrates and did not occur with substrates derived from other retroviruses. Mutagenesis of the U5 long terminal repeat (LTR) terminus revealed only moderate effects of mutations outside the terminal four bases of… CONTINUE READING

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