Substrate specificity of Ca(2+)/calmodulin-dependent protein kinase phosphatase: kinetic studies using synthetic phosphopeptides as model substrates.

@article{Ishida2001SubstrateSO,
  title={Substrate specificity of Ca(2+)/calmodulin-dependent protein kinase phosphatase: kinetic studies using synthetic phosphopeptides as model substrates.},
  author={Atsuhiko Ishida and Yasushi Shigeri and Yoshiro Tatsu and Yasuko Endo and Isamu Kameshita and Sachiko Okuno and Takako Kitani and Masayo Takeuchi and Noboru Yumoto and Hidehiko Fujisawa},
  journal={Journal of biochemistry},
  year={2001},
  volume={129 5},
  pages={745-53}
}
Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKPase) dephosphorylates and regulates multifunctional Ca(2+)/calmodulin-dependent protein kinases. In order to elucidate the mechanism of substrate recognition by CaMKPase, we chemically synthesized a variety of phosphopeptide analogs and carried out kinetic analysis using them as CaMKPase substrates. This is the first report using systematically synthesized phosphopeptides as substrates for kinetic studies on substrate specificities of… CONTINUE READING

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