Substrate specificity of cucumisin on synthetic peptides.

  title={Substrate specificity of cucumisin on synthetic peptides.},
  author={Hiroo Yonezawa and Hiroshi Kaizuka and Tetsuya Uchikoba and Kazunari Arima and Miyuki Kaneda},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={64 10},
The substrate specificity of cucumisin [EC] was identified by the use of the synthetic peptide substrates Leu(m)-Pro-Glu-Ala-Leu(n) (m = 0-4, n = 0-3). Neither Pro-Glu-Ala-Leu (m = 0) nor Leu-Pro-Glu-Ala (n = 0) was cleaved by cucumisin, however other analogus peptides were cleaved between Glu-Ala. The hydrolysis rates of Leu(m)-Pro-Glu-Ala-Leu increased with the increase of m = 1 to 2 and 3, but was however, essentially same with the increase of m = 3 to 4. Similarly, the hydrolysis… CONTINUE READING