Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid

Abstract

An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regio-specificity for C16 and C18 cis-9 unsaturated fatty acids. These fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. Among the cis-9-unsaturated fatty acids, S. nitritireducens showed the highest specificity for linoleic acid. The cells converted 20 mM linoleic acid to 13.5 mM 10-hydroxy-12(Z)-octadecenoic acid at 30°C and pH 7.5 with a yield of 67.5% (mol/mol).

DOI: 10.1007/s00253-007-1280-6

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@article{Yu2007SubstrateSO, title={Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid}, author={In-Sik Yu and Soo-Jin Yeom and Hye-Jung Kim and Jung-Kul Lee and Yong-hwi Kim and Deok-Kun Oh}, journal={Applied Microbiology and Biotechnology}, year={2007}, volume={78}, pages={157-163} }