Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue.

@article{Oehler2002SubstrateSA,
  title={Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue.},
  author={Christian Oehler and J{\"u}rgen Kopitz and Michael Cantz},
  journal={Biological chemistry},
  year={2002},
  volume={383 11},
  pages={1735-42}
}
A ganglioside-specific sialidase that controls cellular functions such as growth, differentiation, and adhesion has been observed in a variety of cells, but its characterization proved difficult due to firm membrane attachment and lability of the purified enzyme. Here we report on the specificity toward gangliosides and susceptibility to certain inhibitors of a ganglioside sialidase solubilized and purified 5100-fold from human brain. The sialidase removed terminal sialic acids from… CONTINUE READING