Substrate-selective and calcium-independent activation of CaMKII by α-actinin.

  title={Substrate-selective and calcium-independent activation of CaMKII by α-actinin.},
  author={Nidhi Jalan-Sakrikar and Ryan K. Bartlett and Anthony J Baucum and Roger J Colbran},
  journal={The Journal of biological chemistry},
  volume={287 19},
Protein-protein interactions are thought to modulate the efficiency and specificity of Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII) signaling in specific subcellular compartments. Here we show that the F-actin-binding protein α-actinin targets CaMKIIα to F-actin in cells by binding to the CaMKII regulatory domain, mimicking CaM. The interaction with α-actinin is blocked by CaMKII autophosphorylation at Thr-306, but not by autophosphorylation at Thr-305, whereas… CONTINUE READING