Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis.

@article{Schoborg2014SubstrateRA,
  title={Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis.},
  author={Jennifer A Schoborg and C. Eric Hodgman and Mark J Anderson and Michael C. Jewett},
  journal={Biotechnology journal},
  year={2014},
  volume={9 5},
  pages={630-40}
}
Cell-free protein synthesis (CFPS) platforms are now considered a powerful tool for synthesizing a variety of proteins at scales from pL to 100 L with accelerated process development pipelines. We previously reported the advancement of a novel yeast-based CFPS platform. Here, we studied factors that cause termination of yeast CFPS batch reactions. Specifically, we characterized the substrate and byproduct concentrations in batch, fed-batch, and semi-continuous reaction formats through high… CONTINUE READING