Substrate recognition domains as revealed by active hybrids between the D-arabinitol and ribitol transporters from Klebsiella pneumoniae

  title={Substrate recognition domains as revealed by active hybrids between the D-arabinitol and ribitol transporters from Klebsiella pneumoniae},
  author={H Heuel and Sevket Turgut and Kurt Schmid and Joseph W. Lengeler},
  journal={Journal of Bacteriology},
  pages={6014 - 6019}
Two new genes, dalT and rbtT, have been cloned from the dal operon for D-arabinitol and the rbt operon for ribitol uptake and degradation, respectively, in Klebsiella pneumoniae 1033-5P14, derivative KAY2026. Each gene codes for a specific transporter which, based on sequence data, belongs to a large family of carbohydrate transporters which constitutes 12 transmembrane helices. DalT and RbtT show an unusually high similarity (86.2% identical residues for totals of 425 and 427 amino acids… 

Genes for D-arabinitol and ribitol catabolism from Klebsiella pneumoniae.

The enzymes for catabolism of the pentitols D-arabinitol (Dal) and ribitol (Rbt) and the corresponding genes from Klebsiella pneumoniae and Escherichia coli have been used intensively in experimental evolutionary studies and showed interesting deviations.

Two class II D-tagatose-bisphosphate aldolases from enteric bacteria

Two D-tagatose 1,6-bisphosphate-specific aldolases involved in catabolism of galactitol and N-acetyl-galactosamine are found in gram-negative and gram-positive bacteria and required subunits GatY or KbaZ for full activity and for good in vivo and in vitro stability.

Transport and Catabolism of Pentitols by Listeria monocytogenes

It is shown that transposon insertion into Listeria monocytogenes lmo2665, which encodes an EIIC of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), is also required for D-xylitol utilization, and this protein is called EIICAxl.

Characterization of the mannitol catabolic operon of Corynebacterium glutamicum

The newly discovered operon comprises the three basic functional elements required for mannitol utilization: regulation, transport, and metabolism to fructose, further processed to the common intermediate of glycolysis fructose-6-phosphate.

Utilization of d-Ribitol by Lactobacillus casei BL23 Requires a Mannose-Type Phosphotransferase System and Three Catabolic Enzymes

An 11-kb region in the genome sequence of L. casei strain BL23 is identified which is absent from strain ATCC 334 and which contains the genes for a GlpR/IolR-like repressor, the four components of a mannose-type PTS, and six metabolic enzymes potentially involved in D-ribitol metabolism.

The genes and enzymes for the catabolism of galactitol, D-tagatose, and related carbohydrates in Klebsiella oxytoca M5a1 and other enteric bacteria display convergent evolution

Results strongly indicate that these various gene clusters and metabolic pathways have been subject to convergent evolution among the Enterobacteriaceae.

Mutations Which Uncouple Transport and Phosphorylation in the d-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14

Interestingly, in the presence of all II(Mtl) variants, growth occurred on the low-affinity analogue D-arabinitol with good efficiency, while only the uncoupled mutated forms transported mannitol at a high rate.

The gal Genes for the Leloir Pathway ofLactobacillus casei 64H

Northern blot analysis showed that the gal genes constitute an operon that is transcribed from two promoters that is inducible by galactose in the medium, whilegalEp constitutes a semiconstitutive promoter located ingalK.

Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

The results show that the XylB, RbtT, and MtlD proteins allow the growth of C. glutamicum on D-arabitol and that D-Arabitol metabolism is subject to arabitol-dependent derepression by AtlR.



Close Genetic Linkage of the Determinants of the Ribitol and d-Arabitol Catabolic Pathways in Klebsiella aerogenes

Klebsiella aerogenes strain W70 has separate inducible pathways for the degradation of the pentitols ribitol and d-arabitol, and the growth of dalC31 is inhibited by xylitol, but the toxicity can be reduced by increasing the levels of ribitol dehydrogenase either by induction with ribitol or by selection of a ribitol dehydrationrogenase-constitutive mutation.

The Structure and Control of the Pentitol Operons

Detailed study of the structure of this region might elucidate the nature of events leading to the enzyme superproduction discussed in the previous chapter, the control of the Operons, and potential homologies between the corresponding rbt and dal genes.

Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism

The gat genes involved in galactitol metabolism have been isolated from the wild-type isolate Escherichia coli EC3132 and cloned on a 7.8-kbp PstI DNA fragment and are expressed constitutively in both strains, probably due to a mutation(s) in gatR.

d-Arabitol Catabolic Pathway in Klebsiella aerogenes

Klebsiella aerogenes strain W70 has an inducible pathway for the degradation of d-arabitol which is comparable to the one found in Aerobacter aerogene strain PRL-R3, and mutants constitutive for ADH were able to use this enzyme to convert the hexitol d-mannitol to d-fructose.

Mammalian and bacterial sugar transport proteins are homologous

The sequences of the arabinose-H+ and xylose- H+ membrane transport proteins of Escherichia coli are determined and are homologous with each other and with the glucose transporters of human hepatoma1 and rat brain2 cells.

Inverted repeats surround the ribitol–arabitol genes of E. coli C

It is reported that the ribitol-arabitol genes of E. coli C are surrounded by 1.4 kilobase (kb) inverted repeats of imperfect homology, which may constitute an example of a transposable element recently acquired by the bacterial chromosome.

The transmembrane topology of the sn‐glycerol‐3‐phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions

The Escherichia coli glpT gene encodes a transport protein that mediates uptake of sn‐glycerol‐3‐phosphate, a member of a class of bacterial organophosphate permeases which transport substrates by antiport with inorganic phosphate, and a two‐dimensional model for the permease is tested.

Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria

Sucrose‐positive derivatives of Escherichia coli K‐12 and Klebsiella pneumoniae hydrolyse intracellular sucrose 6‐phosphate by means of an invertase into d‐glucose 6‐ phosphate and free d‐fructose and show overall identity among each other and to a kinase from Vibrio alginoiyticus also involved in sucrose metabolism.

The Role of Tryptophans 371 and 395 in the Binding of Antibiotics and the Transport of Sugars by the D-Galactose-H+ Symport Protein (GalP) from Escherichia coli(*)

The interactions between the D-galactose-H+ symporter (GalP) from Escherichia coli and the inhibitory antibiotics, cytochalasin B and forskolin, and the substrates, D-galactose and H+, have been