Substrate recognition domains as revealed by active hybrids between the D-arabinitol and ribitol transporters from Klebsiella pneumoniae

@article{Heuel1997SubstrateRD,
  title={Substrate recognition domains as revealed by active hybrids between the D-arabinitol and ribitol transporters from Klebsiella pneumoniae},
  author={H Heuel and Sevket Turgut and Kurt Schmid and Joseph W. Lengeler},
  journal={Journal of Bacteriology},
  year={1997},
  volume={179},
  pages={6014 - 6019}
}
Two new genes, dalT and rbtT, have been cloned from the dal operon for D-arabinitol and the rbt operon for ribitol uptake and degradation, respectively, in Klebsiella pneumoniae 1033-5P14, derivative KAY2026. Each gene codes for a specific transporter which, based on sequence data, belongs to a large family of carbohydrate transporters which constitutes 12 transmembrane helices. DalT and RbtT show an unusually high similarity (86.2% identical residues for totals of 425 and 427 amino acids… 
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Genes for D-arabinitol and ribitol catabolism from Klebsiella pneumoniae.

TLDR
The enzymes for catabolism of the pentitols D-arabinitol (Dal) and ribitol (Rbt) and the corresponding genes from Klebsiella pneumoniae and Escherichia coli have been used intensively in experimental evolutionary studies and showed interesting deviations.

Two class II D-tagatose-bisphosphate aldolases from enteric bacteria

TLDR
Two D-tagatose 1,6-bisphosphate-specific aldolases involved in catabolism of galactitol and N-acetyl-galactosamine are found in gram-negative and gram-positive bacteria and required subunits GatY or KbaZ for full activity and for good in vivo and in vitro stability.

Transport and Catabolism of Pentitols by Listeria monocytogenes

TLDR
It is shown that transposon insertion into Listeria monocytogenes lmo2665, which encodes an EIIC of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), is also required for D-xylitol utilization, and this protein is called EIICAxl.

Characterization of the mannitol catabolic operon of Corynebacterium glutamicum

TLDR
The newly discovered operon comprises the three basic functional elements required for mannitol utilization: regulation, transport, and metabolism to fructose, further processed to the common intermediate of glycolysis fructose-6-phosphate.

Utilization of d-Ribitol by Lactobacillus casei BL23 Requires a Mannose-Type Phosphotransferase System and Three Catabolic Enzymes

TLDR
An 11-kb region in the genome sequence of L. casei strain BL23 is identified which is absent from strain ATCC 334 and which contains the genes for a GlpR/IolR-like repressor, the four components of a mannose-type PTS, and six metabolic enzymes potentially involved in D-ribitol metabolism.

The genes and enzymes for the catabolism of galactitol, D-tagatose, and related carbohydrates in Klebsiella oxytoca M5a1 and other enteric bacteria display convergent evolution

TLDR
Results strongly indicate that these various gene clusters and metabolic pathways have been subject to convergent evolution among the Enterobacteriaceae.

Mutations Which Uncouple Transport and Phosphorylation in the d-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14

TLDR
Interestingly, in the presence of all II(Mtl) variants, growth occurred on the low-affinity analogue D-arabinitol with good efficiency, while only the uncoupled mutated forms transported mannitol at a high rate.

The gal Genes for the Leloir Pathway ofLactobacillus casei 64H

TLDR
Northern blot analysis showed that the gal genes constitute an operon that is transcribed from two promoters that is inducible by galactose in the medium, whilegalEp constitutes a semiconstitutive promoter located ingalK.

Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli.

Arabitol Metabolism of Corynebacterium glutamicum and Its Regulation by AtlR

TLDR
The results show that the XylB, RbtT, and MtlD proteins allow the growth of C. glutamicum on D-arabitol and that D-Arabitol metabolism is subject to arabitol-dependent derepression by AtlR.

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TLDR
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TLDR
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TLDR
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Chapter 10 The lactose permease of Escherichia coli: Past, present and future

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